I'm a biotechnologist who has trained in genomic science, with over 10 years of experience in non-coding RNA Biology. My main interest is in the role of ncRNAs in differentiation and in cancer and, more specifically, in their ability to modify the expression of genes, shaping cell fate. My scientific approach is characterized by the exploitation of genome-wide approaches, novel technologies and bioinformatics.
Address
Via Adamello 16, Milano
Research center
CGS@SEMM
About
Education
Title: PhD in Molecular Medicine
Institute: Università di Milano, European School Of Molecular Medicine (SEMM)
Location: Milan
Country: Italy
From: 2007 To: 2010
Title: Laurea Magistrale in Biotecnologie Farmaceutiche
Institute: Università di Milano
Location: Milan
Country: Italy
From: 2000 To: 2006
Skills
ncRNA biology
- Detection and Manipulation of non coding RNAs by CRISPRi/a/KO, GAPMERs, shRNA, etc.
Single Cell/Molecule Sequencing
- sc-RNA-seq
- sc-ATAC-seq
CRISPR functional Screenings
- CROP-SEQ
- Cell Tracking
Cancer Biology
- Breast Cancer models
- Cancer stem cells
- 3D cell culture
Next Gen Seq
- small RNA-seq
- RNA-seq
- ATAC-seq
Top Publications
2024
Nadalin F., Marzi M.J., Pirra Piscazzi M., Fuentes-Bravo P., Procaccia S., Climent M., Bonetti P., Rubolino C., Giuliani B., Papatheodorou I., Marioni J.C., Nicassio F.
Multi-omic lineage tracing predicts the transcriptional, epigenetic and genetic determinants of cancer evolution
Nature Communications, vol. 15, (no. 1)
Article
Journal
2022
Simeone I., Rubolino C., Noviello T.M.R., Farinello D., Cerulo L., Marzi M.J., Nicassio F.
Prediction and pan-cancer analysis of mammalian transcripts involved in target directed miRNA degradation
Nucleic Acids Research, vol. 50, (no. 4), pp. 2019-2035
2018
Ghini F., Rubolino C., Climent M., Simeone I., Marzi M.J., Nicassio F.
Endogenous transcripts control miRNA levels and activity in mammalian cells by target-directed miRNA degradation
Nature Communications, vol. 9, (no. 1)
2016
Marzi M.J., Ghini F., Cerruti B., De Pretis S., Bonetti P., Giacomelli C., Gorski M.M., Kress T., Pelizzola M., Muller H., Amati B., Nicassio F.
Degradation dynamics of micrornas revealed by a novel pulse-chase approach
Genome Research, vol. 26, (no. 4), pp. 554-565
2015
Montani F., Marzi M.J., Dezi F., Dama E., Carletti R.M., Bonizzi G., Bertolotti R., Bellomi M., Rampinelli C., Maisonneuve P., Spaggiari L., Veronesi G., Nicassio F., Di Fiore P.P., Bianchi F.
MiR-test: A blood test for lung cancer early detection
Journal of the National Cancer Institute, vol. 107, (no. 6)
